Journal of Environmental Biology

Modulatory effects of garlic extract against the cyclophosphamide

induced genotoxicity in human lymphocytes in vitro

 

?B. Lakshmi Sowjanya*, K. Rudrama Devi and D. Madhavi

Department of Zoology, Osmania University, Hyderabad - 500 007, India

(Received: October 15, 2007; Revised received: April 30, 2008; Accepted: July 28, 2008)

 

Abstract: Cyclophosphamide (CP) is a commonly used chemotherapeutic and immunosuppressive agent which is used in the treatment of wide range of cancers and autoimmune diseases. Besides that it is a well known carcinogen. In this study by using chromosomal aberrations (CA) and sister chromatid exchanges (SCE) assays method, the modulatory effects exerted by the extract of garlic against the CP induced genotoxicity in the human lymphocyte cultures in vitro were tested. Three different doses of garlic extract were tested for their modulatory capacity on the mutagenecity exerted by 100 ?g ml^-1 of CP. The results indicate a significant decrease in the frequency of CA and SCE suggesting that the garlic extract modulates the CP induced genotoxicity in a dose dependent manner. These findings provide the future directions for the research on design and development of possible modulatory drugs containing garlic extract.

Key words: Cyclophosphamide, Garlic extract, Genotoxicity, Chromosomal aberrations, Sister chromatid exchanges, Human lymphocytes

 

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