Activity
of phosphatases in the hemocytes
of estuarine edible
mudcrab,
Scylla serrata exposed to arsenic
Sanjib Saha, Mitali Ray and Sajal
Ray*
Aquatic
Toxicology Laboratory, Department of Zoology, University
of Calcutta, Kolkata
- 700 019, India
(Received:
March 03, 2008;
Revised received: September
09, 2008; Accepted: October 10, 2008)
Abstract: Sundarbans biosphere reserve is an ecologically important
area of West Bengal, which supports a wide
variety of estuarine species including Scylla serrata
estuarine mudcrab of economic value. In this present
study, activity of phosphatases (acid and alkaline)
of hemocytes was estimated under the sublethal exposure of arsenic in controlled static water
environment for 1, 2, 3, 4, 7 and 15 days of exposure. Exposure to 3 ppm of sodium arsenite for 15 days, resulted a maximum inhibition of activity of acid phosphatase (ACP) as 0.016 ?M mg-1 protein min-1
with intermediate activity in different spans of exposure against the control
value of 1.83 ?M mg-1 protein min-1. Similar mode of
maximum inhibition of activity of alkaline phosphatase
(ALP) was recorded as 0.008 ?M mg-1 protein min-1,
when the crabs were exposed to 3 ppm of sodium arsenite for 15 days against the control value of 0.694 ?M
mg-1 protein min-1. Exposure to 1 ppm
of sodium arsenite caused inhibition of activity of
ACP which ranged from 1.76 to 0.70 ?M mg-1 protein min-1.
For 2 and 3 ppm of exposure activity of ACP ranged
from 1.12 to 0.42 and 0.67 to 0.016 ?M mg-1
protein min-1 respectively. For the activity of ALP exposure
to 2 and 3 ppm of sodium arsenite
yielded an inhibition of activity which ranged from 0.472 to 0.279 and 0.275 to 0.008 ?M mg-1 protein min-1
respectively. Arsenic exposure resulted a dose
dependent decrease in the activities of both of the phosphatases
and may be considered as a suitable biomarker of aquatic pollution in Sundarbans mangrove.
Key word: Sodium arsenite, Scylla serrata,
Hemocytes, Phosphatase, Sundarbans
??????? PDF of full length paper
is available online
Copyright ? 2009 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any
form without prior permission. Responsibility regarding the authenticity
of the data, and the acceptability of the conclusions enforced or derived, rest
completely with the author(s).
