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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Sep 2009, 30 (5)                                     Back


Paper_06

Activity of phosphatases in the hemocytes of estuarine edible

mudcrab, Scylla serrata exposed to arsenic

 

Sanjib Saha, Mitali Ray and Sajal Ray*

Aquatic Toxicology Laboratory, Department of Zoology, University of Calcutta, Kolkata - 700 019, India

(Received: March 03, 2008; Revised received: September 09, 2008; Accepted: October 10, 2008)

 

Abstract: Sundarbans biosphere reserve is an ecologically important area of West Bengal, which supports a wide variety of estuarine species including Scylla serrata estuarine mudcrab of economic value. In this present study, activity of phosphatases (acid and alkaline) of hemocytes was estimated under the sublethal exposure of arsenic in controlled static water environment for 1, 2, 3, 4, 7 and 15 days of exposure. Exposure to 3 ppm of sodium arsenite for 15 days, resulted a maximum inhibition of activity of acid phosphatase (ACP) as 0.016 ?M mg-1 protein min-1 with intermediate activity in different spans of exposure against the control value of 1.83 ?M mg-1 protein min-1. Similar mode of maximum inhibition of activity of alkaline phosphatase (ALP) was recorded as 0.008 ?M mg-1 protein min-1, when the crabs were exposed to 3 ppm of sodium arsenite for 15 days against the control value of 0.694 ?M mg-1 protein min-1. Exposure to 1 ppm of sodium arsenite caused inhibition of activity of ACP which ranged from 1.76 to 0.70 ?M mg-1 protein min-1. For 2 and 3 ppm of exposure activity of ACP ranged from 1.12 to 0.42 and 0.67 to 0.016 ?M mg-1 protein min-1 respectively. For the activity of ALP exposure to 2 and 3 ppm of sodium arsenite yielded an inhibition of activity which ranged from 0.472 to 0.279 and 0.275 to 0.008 ?M mg-1 protein min-1 respectively. Arsenic exposure resulted a dose dependent decrease in the activities of both of the phosphatases and may be considered as a suitable biomarker of aquatic pollution in Sundarbans mangrove.

Key word: Sodium arsenite, Scylla serrata, Hemocytes, Phosphatase, Sundarbans

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