Journal of Environmental BiologypISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP |
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Abstract - Issue July 2009, 30 (4) BackAntioxidative
efficiency of Triticum aestivum
L. exposed to chromium stress Surjendu Kumar Dey, Priyanka Priyadarshani
Department
of Environmental Sciences, (Received:
Abstract: Wheat (Triticum aestivum
L. cv.? Sonalika) seedlings
were grown? in presence of? K2Cr2O7 (10,
50 and 100 ppm) for 7 days and growth, total
chlorophyll, activities of? antioxidative enzymes like superoxide
dismutase (SOD; EC 1.15.1.1), catalase
(CAT; EC 1.11.1.6) and guaiacol peroxidase
(POX; EC 1.11.1.7) and lipid peroxidation were
determined in root and shoot tissues. Growth of the seedlings was significantly
(p < 0.05) depressed and at 100 ppm, root
length was reduced by 63% and shoot length by 44% in comparison to the
respective controls.? Total chlorophyll
loss in shoots was about 46% at 10 ppm of K2Cr2O7
which further increased to 80% at 100 ppm. Both in
root and shoot tissues, activities of SOD and CAT declined with increase of
metal in growth medium and it was significant (p < 0.05) even at
lowest concentration of the metal tested.?
But POX activity showed a different trend. In root tissues it was
decreased whereas in shoots, there was many fold increase in the activity
(about 370% over control at 100 ppm).? Malondialdehyde
(MDA) content increased both in root and shoot tissues, but it reached
significant (p < 0.05) level at 50 ppm in
roots and at 100 ppm in shoot tissues.? Even though antioxidative
enzyme activities were not assayed in germinating embryos, inhibition in
germination percentage (by 40% at 100 ppm) and
increase in lipid peroxidation level (by 71% over
control at 100 ppm) were observed in 2-day-old embryos, germinated in presence of K2Cr2O7
(10, 50 and 100 ppm). The results indicated the
imposition of oxidative stress situations both during germination and early
stages of seedling growth by Cr6 stress, which might be one of the
probable reasons behind Cr toxicity in plants. Key words: Antioxidative enzymes, Cr6, Lipid peroxidation,
Wheat ? ??????? PDF of full length paper is available
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