Journal of Environmental BiologypISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP |
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Abstract - Issue Jan 2009, 30 (1) BackRadioprotective effect of lycopene on chromosomal
aberrations (CAs) induced by gamma radiation in human lymphocytes Kultigin Cavusoglu*1 and Emine Yalcin2 1Department of Biology, Faculty of Science and Art, 2Department of Biology, Faculty of Science and Art, (Received:
April 16, 2008; Revised received: August 08, 2008; Accepted: August 11, 2008) Abstract: The present study was carried out to
evaluate the radioprotective role of lycopene on chromosomal aberrations (CAs)
induced by gamma (g) radiation in human lymphocytes. For
this purpose, we used in vitro dose-effect relationship, and correlate these
data with statistical parameters. CAs were evaluated in terms of chromosome break, dicentric, tricentric, acentric, fragment and ring. Mitotic index (MI) and
aberrant metaphase number (AMN) were also calculated. Nine groups of lymphocyte
culture were prepared: (I) Control group did not received radiation or lycopene, (II) positive control; lymphocytes were treated
only with 0.020 mM lycopene,
(III) radiation group; lymphocytes were treated with only 10 Gy g–radiation, (IV) lymphocytes were
treated with 0.001 mM lycopene
before irradiation, (V) lymphocytes were treated with 0.005 mM lycopene before irradiation, (VI)
lymphocytes were treated with 0.010 mM lycopene before irradiation, (VII) lymphocytes were treated
with 0.015 mM lycopene before
irradiation, (VIII) lymphocytes were treated with 0.018 mM lycopene before irradiation, (IX)
lymphocytes were treated with treated with 0.020 mM lycopene before irradiation. The results indicated that all
lycopene-supplemented lymphocytes showed a
lower CAs frequency than lymphocytes in only
radiation treated group. It was seen that lycopene
had a protective effect on CAs particularly at 0.010,
0.015 and 0.018 mM concentrations, but this effect
saturated at 0.020 mM concentration. Besides, MI increased
and AMN decreased depending on applied lycopene
doses. In vitro results showed that the lycopene
supplementation decreases frequency of CAs
and its protective role against chromosome damages induced by
radiation is dose-dependent until a certain stage. Key
words: Aberrant metaphase
number, Chromosomal aberrations, Gamma radiation, Lycopene,
Mitotic index PDF of full length paper is available
with author (*kultigincavusoglu@mynet.com) Copyright © 2009 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any
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of the data, and the acceptability of the conclusions enforced or derived, rest
completely with the author(s). |