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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Nov 2008, 29 (6)                                     Back


Interactions between marine facultative epiphyte Chlamydomonas sp

Effects of plant lectin from cobra lily, Arisaema curvatum Kunth on development of melon fruit fly, Bactrocera cucurbitae (Coq.)

 

?Kuljinder Singh*1, Manpreet Kaur2, Pushpinder J. Rup1 and Jatinder Singh2

1Insect Physiology laboratory, Department of Zoology, Guru Nanak Dev University, Amritsar - 143 005, India

?2Protein laboratory, Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar - 143 005, India

(Received: September 12, 2006 ; Revised received: March 20, 2007 ; Accepted:April 30, 2007)

Abstract: The lectin from tubers of cobra lily, Arisaema curvatum Kunth was purified by affinity chromatography using asialofetuin-linked amino activated porous silica beads. The concentration dependent effect of lectin was studied on second instar larvae (64-72 hr) of Bactrocera cucurbitae (Coq.). The treatment not only resulted in a significant reduction in the percentage pupation and emergence of the adults from treated larvae but it also prolonged the remaining larval development period. A very low LC50 value, 39 mgl-1 of lectin was obtained on the basis of adult emergence using probit analysis. The activity of three hydrolase enzymes (esterases, acid and alkaline phosphatases), one oxidoreductase (catalase) and one group transfer enzyme (GSTs: Glutathione S-transferases) was assayed in second instar larvae under the influence of the LC50 of lectin at increasing exposure intervals (0, 24, 48 and 72 hr). The Arisaema curvatum lectin significantly decreased the activity of all the enzymes except for esterases, where the activity increased as compared to control at all exposure intervals. The decrease in pupation and emergence as well as significant suppression in the activities of two hydrolases, one oxidoreductase and one GST enzyme in treated larvae of B. cucurbitae indicated that this lectin has anti-metabolic effect on the melon fruit fly larvae.?

Key words: ? Arisaema curvatum, Bactrocera cucurbitae, Catalase, Esterases, Lectin, Phosphatase, Transferase

PDF of full length paper is available with author (*singhk24@yahoo.co.in)

 

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