Journal of Environmental BiologypISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP |
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Abstract - Issue Nov 2008, 29 (6) BackEffects of
plant lectin from cobra lily, Arisaema
curvatum Kunth on
development of melon fruit fly, Bactrocera cucurbitae (Coq.) ?Kuljinder
Singh*1, Manpreet Kaur2,
Pushpinder J. Rup1
and Jatinder Singh2 1Insect Physiology laboratory,
Department of Zoology, ?2Protein laboratory, Department of Molecular Biology and Biochemistry,
(Received:
September 12, 2006 ; Revised received: March 20, 2007
; Accepted:April 30, 2007) Abstract: The lectin
from tubers of cobra lily, Arisaema curvatum Kunth was purified by
affinity chromatography using asialofetuin-linked
amino activated porous silica beads. The concentration dependent effect of lectin was studied on second instar
larvae (64-72 hr) of Bactrocera cucurbitae
(Coq.). The treatment not only resulted in a significant reduction in the
percentage pupation and emergence of the adults from treated larvae but it also
prolonged the remaining larval development period. A very low LC50
value, 39 mgl-1 of lectin
was obtained on the basis of adult emergence using probit
analysis. The activity of three hydrolase enzymes (esterases, acid and alkaline phosphatases),
one oxidoreductase (catalase)
and one group transfer enzyme (GSTs: Glutathione S-transferases) was assayed in second instar
larvae under the influence of the LC50 of lectin
at increasing exposure intervals (0, 24, 48 and 72 hr). The Arisaema
curvatum lectin
significantly decreased the activity of all the enzymes except for esterases, where the activity increased as compared to control
at all exposure intervals. The decrease in pupation and emergence as well as
significant suppression in the activities of two hydrolases,
one oxidoreductase and one GST enzyme in treated
larvae of B. cucurbitae indicated that this lectin has anti-metabolic effect on the melon fruit fly
larvae.? Key
words: ? Arisaema curvatum,
Bactrocera cucurbitae, Catalase, Esterases, Lectin, Phosphatase, Transferase PDF of full length paper is available with author (*singhk24@yahoo.co.in) Copyright ? 2008 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any
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of the data, and the acceptability of the conclusions enforced or derived, rest
completely with the author(s). |