Journal of Environmental Biology
pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
Search the Journal web-site through Google:
Abstract - Issue Jul 2008, 29 (4) Back
Isolation of cadmium-induced DNA sequence in microalga Nannochloropsis oculata
Joong Kee Min2, Tae Yeon Rhim2, Hyun Woong Shin1 and Mi Young Lee*2
1Department of Marine
Biotechnology, Soonchunhyang University, Asan, Chungnan - 336-600, Korea
(Received: March 22, 2007; Revised received: November 03, 2007; Accepted: December 26, 2007)
Abstract: The gene induced in response to Cd exposure in microalga Nannochloropsis oculata was examined in this investigation. In order to isolate this gene by using nested PCR, degenerated primers were designed on the basis of highly conserved regions of the amino acid sequences of various phytochelatin synthase. The size of the nested PCR amplification product from Nannochloropsis oculata by using two degenerated primers was measured to be 266 bp. The 266 bp DNA fragment was sequenced, and it might be induced by Cd exposure in microalga Nannochloropsis oculata.
Key words: Cadmium, DNA, Nannochloropsis oculata
PDF of full length paper is available with author (firstname.lastname@example.org)
Copyright ? 2008 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).