Journal of Environmental Biology
pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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Abstract - Issue Jul 2008, 29 (4) Back
Detection of marine
pathogenic bacterial Vibrio
species by multiplex polymerase
Mal Nam Kim* and Hyo Joo Bang
Department of Biology, Sangmyung University, Seoul - 110-743, Korea
(Received: February 03 ‘07; Revised received: October 26 ‘07; Re-revised received: November 05 ‘07; Accepted: November 27 ‘07)
Abstract: Polymerase chain reactions (PCR) were performed to accomplish quick and accurate detection of Vibrio species. The primers prepared with 16S-23S rDNA intergenic spacer (IGS) region exhibited an excellent species-specificity for Vibrio sp and detected Vibrio sp more successfully than the conventional culture method. Multiplex PCR was also fruitful not only for the identification of the 5 standard Vibrio sp simultaneously, but also for the detection of Vibrio sp in the samples collected from the natural environment.
Key words: Vibrio sp, Multiplex PCR, Species-specific primer
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