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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Jul 2008, 29 (4)                                                                                                             Back


Identification of a Taq DNA polymerase inhibitor from the red seaweed
Symphyocladia latiuscula

Hyung Joo Jin1, Mi Young Oh1, Deuk Hee Jin1 and Yong Ki Hong*2

1Faculty of Marine Bioscience and Technology, Kangnung National University, Gangneung 210-702, Korea
2Department of Biotechnology, Pukyong National University, Namku, Busan 608-737, Korea

(Received: March 13, 2007; Revised received: October 26, 2007; Accepted: December 26, 2007)

Abstract: Two inhibitors of Taq DNA polymerase were isolated from the marine red alga Symphyocladia latiuscula. The inhibitors were purified by methanol extraction, molecular fractionation below 3,000 MW, and reverse-phase HPLC. The purified compound SL-1 containing three bromines was identified as 2,3,6-tribromo-4,5-dihydroxybenzyl alcohol (C7H5Br3O3: MW 374) by NMR and MS analyses. The purified compound SL-2 was identified as 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether (C8H7Br3O3: MW 388). In a 25-l reaction mixture containing 1.5 units of Taq DNA polymerase, the enzyme was completely inhibited by 0.5 g SL-1 or 5 g SL-2.

Key words: DNA polymerase inhibitor, Symphyocladia latiuscula, 2,3,6-tribromo-4,5-dihydroxybenzyl alcohol, 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether

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