Journal of Environmental Biology
pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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Abstract - Issue Jul 2008, 29 (4) Back
Identification of a Taq DNA polymerase inhibitor from the red seaweed
Hyung Joo Jin1, Mi Young Oh1, Deuk Hee Jin1 and Yong Ki Hong*2
1Faculty of Marine
Bioscience and Technology, Kangnung National
University, Gangneung 210-702, Korea
(Received: March 13, 2007; Revised received: October 26, 2007; Accepted: December 26, 2007)
Abstract: Two inhibitors of Taq DNA polymerase were isolated from the marine red alga Symphyocladia latiuscula. The inhibitors were purified by methanol extraction, molecular fractionation below 3,000 MW, and reverse-phase HPLC. The purified compound SL-1 containing three bromines was identified as 2,3,6-tribromo-4,5-dihydroxybenzyl alcohol (C7H5Br3O3: MW 374) by NMR and MS analyses. The purified compound SL-2 was identified as 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether (C8H7Br3O3: MW 388). In a 25-µl reaction mixture containing 1.5 units of Taq DNA polymerase, the enzyme was completely inhibited by 0.5 µg SL-1 or 5 µg SL-2.
Key words: DNA polymerase inhibitor, Symphyocladia latiuscula, 2,3,6-tribromo-4,5-dihydroxybenzyl alcohol, 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether
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