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Journal of Environmental BiologypISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP |
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Abstract - Issue Mar 2009, 29 (2) BackSolubilization of water-insoluble β -glucan isolated from Ganoderma lucidum Man Deuk Han1, Yeon Soo
Han2, Sung
Hee Hyun3?and
Hyun Woung Shin*4 1Department of Biology, 2Department of Agricultural Biology, 3Department of Clinical Laboratory Science,
4Department of Marine
Biotechnology, (Received:
December 22, 2006 ; Revised received: June 20, 2007 ; Accepted: June 29, 2007) Abstract:
The fungal β-D-glucan is a biological
response modifier (BRM), but a major obstacle to the clinical utilization of β-glucan BRMs is their relative lack of solubility in aqueous media.
Water insoluble fungal glucans extracted by alkali
from the mycelia of Ganoderma lucidum
were sulfated to yield their corresponding water-soluble derivatives. Insoluble
glucan is dissolved in methyl sulfoxide
and urea, and is partially sulfated with sulfuric acid. The sulfated glucan (SGL) yield prepared from insoluble glucan (IGL) was 85%, the sulfation
degree of SGL was about 14.9%, and the solubility of SGL was above 95% in
water. The monosugar SGL content was 34.9% a-glucose and 35.9% β-glucose. The mean molecular weight (MW) of SGL was
shown as a single peak on Sepharose CL-4B column
chromatography, and their MW was approximately 9.3 kDa.
The 13C NMR spectrum analysis shows that SGL has a
high similarity with the β-(1?3)-linked
triple-helical control. Key
words: β-Glucan, Sulfated glucan, Biological response modifier, Ganoderma
lucidum PDF
of full length paper is available with author (marinebiotechnology@sch.ac.kr) Copyright ? 2008 Triveni Enterprises. All rights reserved. No part of the
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