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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Mar 2009, 29 (2)                                     Back


Ovarian development in Labeo dyocheilus (McClelland) during active

Solubilization of water-insoluble β -glucan isolated from Ganoderma lucidum

Man Deuk Han1, Yeon Soo Han2, Sung Hee Hyun3?and Hyun Woung Shin*4

1Department of Biology, Soonchunhyang University, Asan - 336 745, Chungnam, South Korea

2Department of Agricultural Biology, Chonnam National University, Gwangju - 500 757, South Korea

3Department of Clinical Laboratory Science, Eulji University, Daejon - 301 832, South Korea

4Department of Marine Biotechnology, Soonchunhyang University, Asan - 336 745, Chungnam, South Korea

(Received: December 22, 2006 ; Revised received: June 20, 2007 ; Accepted: June 29, 2007)

Abstract: The fungal β-D-glucan is a biological response modifier (BRM), but a major obstacle to the clinical utilization of β-glucan BRMs is their relative lack of solubility in aqueous media. Water insoluble fungal glucans extracted by alkali from the mycelia of Ganoderma lucidum were sulfated to yield their corresponding water-soluble derivatives. Insoluble glucan is dissolved in methyl sulfoxide and urea, and is partially sulfated with sulfuric acid. The sulfated glucan (SGL) yield prepared from insoluble glucan (IGL) was 85%, the sulfation degree of SGL was about 14.9%, and the solubility of SGL was above 95% in water. The monosugar SGL content was 34.9% a-glucose and 35.9% β-glucose. The mean molecular weight (MW) of SGL was shown as a single peak on Sepharose CL-4B column chromatography, and their MW was approximately 9.3 kDa. The 13C NMR spectrum analysis shows that SGL has a high similarity with the β-(1?3)-linked triple-helical control.

Key words: β-Glucan, Sulfated glucan, Biological response modifier, Ganoderma lucidum

PDF of full length paper is available with author (marinebiotechnology@sch.ac.kr)

 

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