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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Jan 2008, 29 (1)                                                                                                             Back



paper

Agricultural waste from the tequila industry as substrate for the production of commercially important enzymes

C. Huitron*1, R. Perez1, A.E. Sanchez1, P. Lappe2 and L. Rocha Zavaleta1

1Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Circuito Escolar S/N, Ciudad Universitaria, Apdo. Postal 10228, Mexico D.F. C.P. - 04510, Mexico

2Departamento de Botanica, Instituto de Biologia, Universidad Nacional Autonoma de Mexico, 3er. Circuito S/N,

Ciudad Universitaria, Mexico D.F. C.P. - 04510, Mexico

(Received: November 06, 2006 ; Revised received: January 15, 2007 ; Accepted: June 03, 2007)

Abstract: Approximately 1 million tons of Agave tequilana plants are processed annually by the Mexican Tequila industry, generating vast amounts of agricultural waste. The aim of this study was to investigate the potential use of Agave tequilana waste as substrate for the production of commercially important enzymes. Two strains of Aspergillus niger (CH-A-2010 and CH-A-2016), isolated from agave fields, were found to grow and propagate in submerged cultures using Agave tequilana waste as substrate. Isolates showed simultaneous extracellular inulinase, xylanase, pectinase, and cellulase activities.? Aspergillus CH-A-2010 showed the highest production of inulinase activity (1.48 U/ml), whereas Aspergillus niger CH-A-2016 produced the highest xylanase (1.52 U/ml) and endo-pectinase (2.7 U/ml) activities. In both cases production of enzyme activities was significantly higher on Agave tequilana waste than that observed on lemon peel and specific polymeric carbohydrates. Enzymatic hydrolysis of raw A. tequilana stems and leaves, by enzymes secreted by the isolates yielded maximum concentrations of reducing sugars of 28.2 g/l, and 9.9 g/l respectively. In conclusion, Agave tequilana waste can be utilized as substrate for the production of important biotechnological enzymes.

Key words:???? Agave tequilana, Microbial enzymes, Agave waste, Aspergillus niger

 

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