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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Oct 2007, 28 (4)                                     Back


Cytochrome P450 1Aactivity in liver and fixed wavelength fluorescence detection

of polycyclic aromatic hydrocarbons in the bile of tongue-fish (Cynoglossus acrolepidotus, Bleeker) in relation to petroleum hydrocarbonsin the eastern Gulf of Thailand


Voravit Cheevaporn*1 and F. William H. Beamish2


1Department of Aquatic Science, Faculty of Science, Burapha University, Chonburi-20131, Thailand

2Department of Biology, Faculty of Science, Burapha University, Chonburi-20131, Thailand

(Received: June 15, 2006 ; Revised received: September 20, 2006 ; Accepted: October 20, 2006)

Abstract: This investigation was conducted in an area of oil spill along the east coast of Thailand to examine the relations among cytochrome P450 1A activity in liver andPAHs in the bile of the tonguefish and petroleum hydrocarbons in the sediments. PAH sediment concentrations in the reference and oil spill areas were 5.03 + 0.42 and 0.21 + 0.043mg-1 dry weightrespectively. Cytochrome activity in fish liver from oil spill area was 45.40 + 3.50 pmoles/min/mg protein, almost threefold higher than that from the reference sites. Flourescense detection in bile metabolites at the oil spill area, 69.8 + 9.9 flourescense unitwas significantly higher than that at the reference sites, 22.9 + 5.5 and 22.2 + 3.5 fluorescence unit. A strong correlation was found among cytochrome P450 1A activity in liver, PAH of bile metabolites and petroleum hydrocarbons. Both cytochrome and bile metabolites activity decreased seaward varying to the distance from the oil polluted area. We concluded that both detections in tonguefish can be regarded as a complementary biomarkers for theexposure of PAHs in tropical marine environments.

Key words: Cytochrome P 450 1A, Fixed wavelength fluorescence, Polycyclic aromatic hydrocarbon, Tongue-fish

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