Journal of Environmental Biology
pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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Abstract - Issue Jul 2007, 28 (3) Back
Effect of Pseudomonas aeruginosa on the giant freshwater prawn, Macrobrachium rosenbergii - histopathological and electron microscopic study
K. Ramalingam*1 and
1PG (Aquaculture) and Research Department of Advanced Zoology and Biotechnology,
(Received: August 04, 2005 ; Revised received: February 10, 2006 ; Accepted: March 29, 2006)
Abstract: The giant freshwater prawn Macrobrachium rosenbergii was injected with an inoculum containing LD50 96 hr dose of 107 Pseudomonas aeruginosa (MTCC 1688) to determine the histopathological effects in vivo. The comparison of tissues of both the control and the bacterial endotoxin treated prawns after 96 hr revealed significant degenerative changes in treated prawns. Both light microscopic and electron microscopic observations revealed the infiltration of the tissues of Pseudomonas sp in the muscular and hepatopancreatic tissues of prawn. The muscular tissue changes in the myofibrillar arrangement with blockage at the gap junctions and necrotic lesions were observed. The hepatopancreatic cells were vacuolated with hypertrophied nucleus. Atrophy of hepatopancreatic tubules was conspicuous. The pathogenicity of Pseudomonas aeruginosa is attributed to its infiltration and multiplication inside the tissues and the consequent release of extra-cellular enzymes for its metabolism. The degeneration of host tissues is also attributed to the latter.
Key words: Macrobrachium rosenbergii, Pseudomonas aeruginosa, Extracellular proteases, Endotoxin
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