JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status

Search the Journal web-site through Google:

        Abstract - Issue Jul 2007, 28 (3)                                                                                                             Back


Effect of Pseudomonas aeruginosa on the giant freshwater prawn, Macrobrachium rosenbergii - histopathological and electron microscopic study

K. Ramalingam*1 and S. Ramarani2

1PG (Aquaculture) and Research Department of Advanced Zoology and Biotechnology,

Government Arts College, Nandanam, Chennai-600 035, India

2Chellamal College for Women, Guindy, Chennai-600 035, India

(Received: August 04, 2005 ; Revised received: February 10, 2006 ; Accepted: March 29, 2006)

Abstract: The giant freshwater prawn Macrobrachium rosenbergii was injected with an inoculum containing LD50 96 hr dose of 107 Pseudomonas aeruginosa (MTCC 1688) to determine the histopathological effects in vivo. The comparison of tissues of both the control and the bacterial endotoxin treated prawns after 96 hr revealed significant degenerative changes in treated prawns. Both light microscopic and electron microscopic observations revealed the infiltration of the tissues of Pseudomonas sp in the muscular and hepatopancreatic tissues of prawn. The muscular tissue changes in the myofibrillar arrangement with blockage at the gap junctions and necrotic lesions were observed. The hepatopancreatic cells were vacuolated with hypertrophied nucleus. Atrophy of hepatopancreatic tubules was conspicuous. The pathogenicity of Pseudomonas aeruginosa is attributed to its infiltration and multiplication inside the tissues and the consequent release of extra-cellular enzymes for its metabolism. The degeneration of host tissues is also attributed to the latter.

Key words: Macrobrachium rosenbergii, Pseudomonas aeruginosa, Extracellular proteases, Endotoxin

PDF file of full length paper is available with author


Copyright 2007Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).