Journal of Environmental Biology

Effect of cold stratification treatments on germination of drought tolerant shrubs seeds

 

Zafer Olmez*¹, Fatih Temel¹, Askin Gokturk¹ and ?Zeki Yahyaoglu²

¹Artvin Faculty of Forestry, Kafkas University, Artvin-08000, Turkey

²Faculty of Forestry, Karadeniz Technical University, Trabzon-61080, Turkey

(Received: March 18, 2006 ; Revised received: August 11, 2006 ; Accepted: September 20, 2006)

Abstract: This study was carried out to determine effects of different durations (20, 40, 60 days and control) of cold stratification treatments on seed germination and to overcome dormancy in seeds of twelve different drought tolerant plants. The species used in this study were Arbutus andrachne L., Cistus creticus L., Colutea armena Bois. Huet., Cotinus coggygria Scop., Cotoneaster numullaria Fisch. and Mey., Elaeagnus angustifolia L., Jasminum fruticans L., Paliurus spina-christii Mill., Punica granatum L., Pyracantha coccinea Roem., Rhus coriaria L., and Ziziphus jujuba Mill. The seeds were sown in polyethylene pots under greenhouse and on seedbeds under open field conditions. Statistical design was a randomized complete block design with three replications. Germinated seeds were counted and observed periodically for 90 days after sowing to determine germination percentages (GP) and germination rates (GR). While the highest GP were determined according to duration of cold stratification under greenhouse conditions for each species were 44.2% for 60 days, 2.5% for 20 days, 85.6% for 60 days, 13.5% for 20 and 60 days, 64.3% for 20 days, 11.2% for 60 days, 8.2% for 20 days and 14.1% for 20 days for Cotinus coggygria, Cotoneaster numullaria, Jasminum fruticans, Paliurus spina-christii, Pyracantha coccinea, Punica granatum, Rhus coriaria and Ziziphus jujuba respectively. The highest GP were 16.5% for Arbutus andrachne and 91.3% for Colutea armena for 20 days cold stratified seeds and sown under open field conditions. Furthermore, the highest GP were obtained from seeds of Cistus creticus (27.5%) and Elaeagnus angustifolia (56.2%) in control sowing

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