JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status

Search the Journal web-site through Google:

        Abstract - Issue Apr Supplement 2007, 28 (2)                                                                                                             Back


Response of wheat seed germination and seedling growth under copper stress

Dharam Singh, Kamlesh Nath and Yogesh Kumar Sharma*

Department of Botany, University of Lucknow, Lucknow-226 007, India

(Received: May 25, 2005 ; Revised received: November 01, 2005 ; Accepted: January 25, 2006)

Abstract: The experiment was performed to study the seed germination and seedling growth in wheat under the influence of different concentrations of copper. The germination %, plumule and radicle length, and number of lateral roots decreased with increase in copper concentration (5, 25, 50 and 100 mgl-1). Total chlorophyll contents declined on 14th day from 1.605 of control to 1.581, 1.242, 1.275 and 1.107 mg g-1 fresh weight in respective treatments. Similarly, on 21st day the decline in total chlorophyll contents was 1.288, 1.123, 1.077 and 0.985 mg g-1 fresh weight in respective treatments against 1.724 of control. Likewise the pheophytin contents also declined showing the same pattern. However, carotenoid contents increased in different treatments, ranging between 0.366 to 0.464 mg g-1 fresh weight in comparison to control (0.328) on 14th day, but showed adverse effects on 21st day as the carotenoid contents decreased in different copper treatments. The activity of amylase was found to be gradually reduced 14th day from 29.73 of control to 27.80, 27.33, 21.86 and 20.00 mg g-1 and at 21st day from 14.40 of control to 11.46, 11.01, 9.86 and 5.60 mg g-1 fresh weight with increase in concentrations of copper. The catalase activity increased 14th day from 97.33 of control to 134.66, 161.33, 216.00 and 232.00 and on 21st day from 140.00 of control to 245.33, 274.66, 278.66 and 300.66 ml H2O2 hydrolyzed/g fresh weight in different increased concentrations of copper. Similarly, the peroxidase activity was also increased with increase in copper concentration. Likewise fresh weight and moisture contents decreased with increase in copper concentration. The dry weight was increased with increase in concentration of copper treatment. Total protein contents were initially decreased on 14th day from 80.69 of control to 66.75, 60.41, 56.41 and 48.48 mg/mg and on 21st day 81.37 of control to 67.06, 62.31, 54.92 and 46.47 mg/mg fresh weight in different copper concentrations respectively. Sugar contents were significantly decreased in all the doses of copper on both 14th and 21st days i.e. (5.53 of control to 4.76, 3.69, 3.68 and 2.86 mg/mg in different copper treatments on 14th day and 4.81 of control to 4.49, 3.40, 2.79 and 2.15 mg/mg on 21st day respectively.

Key words: Copper, Chlorophyll, Pheophytin, Carotenoid, Amylase, Catalase, Peroxidase


Copyright 2007 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).