Morphometric and electrophoretic
analysis of 13 populations of Anatolian black pine in Turkey
Ibrahim Turna1, Zeki Yahyaoglu1, Filiz
Y?ksek1, F. Ahmet Ayaz2 and Deniz Guney1
1Department
of Forest Engineering and 2Department
of Biology,
?Karadeniz
Technical University,
61080- Trabzon,
Turkey
(Received:
07 August, 2004 ; Accepted: 20 July, 2005)
Abstract: The genetic variation in populations of
Anatolian black pine (Pinus nigra
Arn. subsp. pallasiana (L.) Holmboe.), one of
the species covering large areas in Turkey, was investigated. Open
pollinated seeds were collected from 13 populations in a natural distribution
range. Six characters of seeds (length, width, ratio of length to width,
weight/1000 seeds) and seedling characters (cotyledon number and hypocotyls
height) and two enzyme systems viz. leucine aminopeptidase (LAP) and glutamate oxaloacetate
transaminase, (GOT) were investigated. Significant
differences were detected among the populations for the morphological
characters. In addition, isozyme patterns of two
enzyme systems revealed that LAP has two loci (one with 2 alleles and the other
with 3), while GOT has three loci (two with 3 alleles and the third one with 2
alleles). Polymorphic loci were 74% on the average. The mean number of alleles
per loci was 1.94 and expected heterozygosity was
19%. The mean total genetic diversity was calculated
as 0.203; the mean gene diversity within populations was determined as 0.188,
and the average between subpopulations diversity was 0.016. The relative magnitude
of genetic differentiation among subpopulations was measured as 0.074
indicating that only 7.4% of the total genetic diversity was there between
populations. Average genetic distance was 0.093 according to Gregorius. Nei's genetic distance
was 0.022.
Key words:
Pinus nigra, Diversity, Isozymes, Endosperm, Seed, Seedling.
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