nitrate-related compounds on growth, survival and hematological responses in tadpoles
of the Cuban tree frog, Osteopilus septentrionalis? (Boulenger)
F. Punzo1? and?
1 Department of Biology, Box 5F,
University of Tampa, Tampa, Florida ?33606, USA
Box 546, Tampa, Florida 33606, USA
(Received: 16 April, 2004 ; Accepted: 08 February, 2005)
Abstract: Experiments were conducted to
assess the effects of nitrate-related compounds on survival, growth, and
hematological responses in tadpoles of the Cuban treefrog,
Osteopilus septentrionalis . Stage-25 tadpoles were exposed to a nitrate dilution
series and exposed to distilled water (controls), 40 ppm
nitrate, or 100 ppm nitrate. Survivorship was
significantly higher for control animals as compared to those exposed to 40 and
100 ppm nitrate. Total blood Hb
concentrations were not significantly altered by exposure to sodium nitrite,
and a significant positive correlation was found between methemoglobinemia
and nitrite concentration over the test range of 1.0 to 50.0 mg/l. Percentage Hb was significantly correlated with nitrite concentration.
Percentage MHb for all treatment groups was
significantly higher (18.4 to 45.3 %) than that of controls (5.4 %).
Key words:? Growth, Hematological responses, Nitrate, Osteopilus.
? 2006 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any
form without prior permission. Responsibility regarding the authenticity
of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).