Journal of Environmental Biology
pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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Abstract - Issue Apr 2006, 27 (2) Back
Sphingomonas paucimobilis is capable of modifying polycyclic aromatic compounds present at sub-nanomolar concentrations in a complex medium
Douglas B. Craig, Heather L. Ash and Juli T. Nachtigall
(Received: 1 March, 2005 ; Accepted: 27 September, 2005)
Abstract: Sphingomonas paucimobilis was incubated in a complex medium amended with 95 pM to 950 nM fluorescein or resorufin. The samples were centrifuged and the supernatants analyzed by capillary electrophoresis utilizing laser-induced fluorescence detection. The growing cells failed to affect the fluorescein concentration but decreased the resorufin concentration by approximately 90% at all concentrations tested. The decrease in resorufin was due to conversion into a non-fluorescent product rather than adsorption to the cells.
Key words: Biodegradation, Capillary electrophoresis, Nanomolar, Polycyclic aromatic, Sphingomonas paucimobilis.
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