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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Apr 2006, 27 (2)                                                                                                             Back



paper

Sphingomonas paucimobilis is capable of modifying polycyclic aromatic compounds present at sub-nanomolar concentrations in a complex medium

 

Douglas B. Craig, Heather L. Ash and Juli T. Nachtigall

Chemistry Department, University of Winnipeg, Winnipeg, MB, Canada, R3B 2E9

 

(Received: 1 March, 2005 ; Accepted: 27 September, 2005)

 

Abstract: Sphingomonas paucimobilis was incubated in a complex medium amended with 95 pM to 950 nM fluorescein or resorufin. The samples were centrifuged and the supernatants analyzed by capillary electrophoresis utilizing laser-induced fluorescence detection. The growing cells failed to affect the fluorescein concentration but decreased the resorufin concentration by approximately 90% at all concentrations tested. The decrease in resorufin was due to conversion into a non-fluorescent product rather than adsorption to the cells.

 

Key words: Biodegradation, Capillary electrophoresis, Nanomolar, Polycyclic aromatic, Sphingomonas paucimobilis.

 

 

 

 

 

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