Journal of Environmental BiologypISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP |
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Abstract - Issue Apr 2006, 27 (2) BackSphingomonas paucimobilis
is capable of modifying polycyclic aromatic compounds present at sub-nanomolar concentrations in a complex medium Douglas B. Craig, Heather L. Ash
and Juli T. Nachtigall Chemistry Department, (Received:
1 March, 2005 ; Accepted: 27 September, 2005) Abstract: Sphingomonas paucimobilis
was incubated in a complex medium amended with 95 pM
to 950 nM fluorescein or resorufin. The samples were centrifuged and the supernatants
analyzed by capillary electrophoresis utilizing laser-induced fluorescence
detection. The growing cells failed to affect the fluorescein
concentration but decreased the resorufin
concentration by approximately 90% at all concentrations tested. The decrease
in resorufin was due to conversion into a
non-fluorescent product rather than adsorption to the cells. Key words: Biodegradation, Capillary electrophoresis, Nanomolar, Polycyclic
aromatic, Sphingomonas
paucimobilis. Copyright
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