Journal of Environmental Biology

Physico-chemical characterization of β-Glucan produced from

Bacillus cereus LVK13 (KC 898956)

Lakshminarayanan Vijayakumar¹* and Ponnusamy Ramalingam²

¹Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam - 638401, India

²Department of Biotechnology, Kumaraguru College of Technology, Coimbatore - 641049, India

*Corresponding Author E-mail: lrvijayakumaar@gmail.com

Publication Data

Paper received:

21 September 2015

Revised received:

26 May 2016

Accepted:

24 August 2016

Abstract

The present study aimed to produce and characterize β-glucan from an indigenous soil isolate Bacillus cereus LVK13 (KC 898956). Thin-layer chromatography resulted an Rf value of 0.58 that corresponded to glucose. Vibrational frequencies of Fourier Transform Infrared Spectroscopy at 2885 cm^-1, 1188 cm^-1 and 887 cm^-1 signified the existence of -CH₂-, C=O bonds and β-configuration. Further, β configuration of monomeric glucose and β-(1,3)-D-glucan backbone were retrieved from the spectrum of ¹H and ¹³C NMR. X-Ray diffraction pattern reflected low crystalline nature of the product. The results of physico-chemical analysis substantiate that β-glucan produced from the indigenous strain Bacillus cereus LVK13 can be used widely in medical, health, food and environmental sectors.   

 Key words

Bacillus cereus LVK13, β-1,3-glucan, FTIR, NMR, XRD

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