Journal of Environmental Biology

Comparison of synthetic dyes decolourisation by Ganoderma sp.

using immobilized enzyme

Prathibha B. Iyer^1,2, B. Atchaya¹, K. Sujatha¹ and K. Rajmohan²

 ¹Department of Chemical Engineering, Dr.M.G.R Educational and Research Institute University, Maduravoyal-600 095, India

²Department of Chemical Engineering, University of Petroleum and Energy Studies, Dehradun-248 007, India

*Corresponding Author E-mail: baskaranpratibha@gmail.com

Publication Data

Paper received:

16 October 2015

Revised received:

09 August 2016

Accepted:

28 August 2016

Abstract

The effluents from textile industries, which are disposed to the open environment and water sources, create major problem in polluting the environment. In Tamil Nadu districts like Tiruppur, people are facing problems due to disposal of untreated textile dyes. Pre-treatment of dyes help in reducing toxic chemicals present in the dye. Various species of white rot fungi are prominent producers of enzyme laccase. In the present study, Ganoderma species were screened isolated for laccase activity major laccase producing fungal strains was identified. In the second phase, the highest amount of laccase producing strain was compared with remaining strains, and the conditions for growth of Ganoderma species was standardized to maximize laccase production. Characterization of enzyme laccase synthesized by Ganoderma species was performed after optimization and partial purification. In the present study, application of characterized laccase in the degradation of textile dyes was investigated. Finally, pH and temperature optimum parameters were upgraded for dye decolorisation and compared to assess the maximum percentage of degradation. The percentage degradation of Direct Blue E was maximum than Acid Violet 4BY and Acid Brilliant Black due to maximum impact of laccase enzyme on that particular Direct Blue E dye.   

 Key words

Dye degradation, Ganoderma sp., Immobilized enzyme, Laccase, White rot fungi

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